Among the most frequently used

methodologies in the field of liquid biopsy we can include:

In order to check if the IHC staining protocol

works, we can skip the primary antibody binding step in the staining protocol. That

way we can evaluate, if there is no nonspecific reaction of the detection

system to the tissue structures. Such sample without primary antibodies is

called „positive control“.

Immunohistochemistry

can be used in clinical practice to determine the pathological diagnosis, to

predict how tumor responds to treatment or to estimate the cancer patients‘

prognosis. Match correct couples (antigen – pathological diagnosis):

Detection of the antigen-antibody complex using the enzymatic method is

based on the fluorescence principle. The antigen-antibody complex can be observed

using fluorescence microscope.

Monoclonal antibodies bind only to one specific epitope of the target

antigen. Therefore, the monoclonal antibodies usually have higher specificity

when compared to the polyclonal antibodies.

The indirect method of

IHC staining has higher sensitivity when compared to the direct method.

Match the micrographs with the organs - step 4

Match the micrographs with the organs - step 5

Match the micrographs with the organs - step 3

Match the micrographs with the organs - step 2