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BIOC2181-Fundamentals of Biochemistry T3 2025
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Effect of cyanide
As you may know from reading detective fiction or watching crime shows, cyanide is a very rapidly acting poison. You suspect that it affects the respiratory chain and so you have designed an experiment to investigate its mechanism of action using techniques that you have just used to invesitgate DNP and MPP+.
The results of your investigation are that upon addition of cyanide, oxygen consumption is inhibited when BOTH glutamate and succinate are used as substrates. From this, you conclude:
Uncouplers
Uncouplers increase the permeability of the inner mitochondrial membrane to protons which allows protons to diffuse back across the membrane and so disrupt the proton gradient.
Based on your experimental observations, the effect of this will be:
Energy Transformation
The major purpose of the electron transport chain is to provide the energy for the synthesis of ATP from ADP and inorganic phosphate (Pi).
The energy is provided by:
Effect of cyanide
As you may know from reading detective fiction or watching crime shows, cyanide is a very rapidly acting poison. You suspect that it affects the respiratory chain and so you have designed an experiment to investigate its mechanism of action using techniques that you have just used to invesitgate DNP and MPP+.
The results of your investigation are that upon addition of cyanide, oxygen consumption is inhibited when BOTH glutamate and succinate are used as substrates. From this, you conclude:
Mechanism of action of MPP+
You should have observed that oxygen consumption resumed upon the addition of succinate. This would suggest that:
(There are two correct answers)
Effect of MPP+
Hopefully, you will have noticed that oxygen consumption ceased when MPP+ was added.
What do you think is the most likely reason for this?
Uncouplers
Uncouplers increase the permeability of the inner mitochondrial membrane to protons which allows protons to diffuse back across the membrane and so disrupt the proton gradient.
Based on your experimental observations, the effect of this will be: