causes thrombocytopenia

blocks all anti-apoptotic Bcl-2 family proteins

is a broad spectrum inactivator of the Bcl2 family of proteins

is more specific for Bcl-2 over Bcl-xl

lysis of the plasma membrane

DAMP protein release

nuclear blebbing

an inflammatory response

GFP will be present in both the cytosol and nucleus

GFP has a positive stretch of amino acids and will therefore be actively delivered into the nucleus.

GFP binds to importin and is delivered to the nucleus

GFP is a large protein and hence cannot move out of the cytosol.

Correct folding of proteins

Addition of mannose-6-phoshate to the proteins

Addition of N-Acetyl galactosamine to the proteins

Phosphorylation of proteins on tyrosine or threonine residues

G2

G1

S

G0

first require phosphorylation in the cytosol at the nuclear localization signal 

contain a nuclear localization signal when they are larger than ~50kDa or for their efficient import when they are less than ~50 kDa

need to be kept in an unfolded conformation by cytosolic chaperones 

are first imported into the ER before binding importin and undergoing vesicle-mediated transport to the nuclear pore 

usually inactive as activation loop masks ATP binding site

have ATP bound

are protein kinases that dephosphorylate proteins

are activated by binding to cyclin

Dynamin (GTPase)

Protein Phosphatase

Protein kinase

Clathrin

Bcl2

OXA

SAM

TIM23

Clathrin

Lipoprotein

Transferrin

Glycoprotein