You have a cell suspension for which you need to determine the cell concentration.

Your counts for each of the outer squares are:

1. 178
2. 181
3. 165
4. 175

What is the cell concentration for your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

You do not need to provide an answer for this question.  Your calculations quiz grade will be inputted here manually.

Donated blood is not transfused as ‘whole blood’, but is separated into separate products, most commonly red blood cells, platelets, and plasma. Plasma separated from whole blood and frozen within 18 hours of collection is known as fresh frozen plasma (FFP).

Fresh frozen plasma can be given in clinical situations where there is active bleeding and abnormal clotting.  FFP from which of the following donors would be suitable for an individual with B+ blood? (Choose all that apply).

(Hint: what immune component is in plasma?)

Which donor could be used as a source of red blood cells for the patient?

Match the patient and donor to the correct blood type.

You need to prepare 750ml of Tyrode's Solution (Acidic).  The components of this solution are:

• 0.1368M Sodium chloride (Mw: 58.44)
• 0.002686M Potassium chloride (Mw: 74.55)
• 0.001632M Calcium chloride dihydrate (Mw: 147.01)
• 0.0004918M Magnesium chloride hexahydrate (Mw: 203.3)
• 0.00555M Glucose (180.16)
• 0.0001M Polyvinylpyrrolidone (Mw: 40000)

You need to prepare 750ml of Lysis solution.  The components of this solution are:

• 2.5M Sodium chloride (Mw: 58.44)
• 100mM Disodium EDTA (Mw: 372.24)
• 10mM Tris base (Mw: 121.14)
• 20mM Sodium hydroxide (Mw: 40.00)
• N-lauroylsarcosine sodium salt (Mw: 293.38)

You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with white cell diluting fluid, using 20μl of cell suspension and 40μl of white cell dilution factor.

Your counts for each of the outer squares are:

1. 56
2. 73
3. 74
4. 70

What is the cell concentration for your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with trypan blue, using 20μl of cell suspension and 40μl of trypan blue.  Trypan blue stains dead cells blue, while live cells exclude this dye.

Your counts for each of the outer squares are:

1. Viable: 83; Dead: 36
2. Viable: 79; Dead: 50
3. Viable: 79; Dead: 48
4. Viable: 86; Dead: 47

What is the percentage of viable cells in your sample?  Answer to 1 decimal place.

You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with trypan blue, using 20μl of cell suspension and 20μl of trypan blue.  Trypan blue stains dead cells blue, while live cells exclude this dye.

Your counts for each of the outer squares are:

1. Viable: 112; Dead: 26
2. Viable: 100; Dead:22
3. Viable: 109; Dead: 28
4. Viable: 107; Dead: 21

What is the concentration of viable cells in your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)