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MCB2011 - Molecular biology and the cell - S1 2025

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When setting up the colorimetric assay, in what order should the sample and reagents be added to each well?

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The assay probe has been provided at a stock concentration of 50µg/mL.

Calculate how many µL of probe must be diluted to prepare 1mL of a

1µg/mL solution.

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The phenomenon whereby Escherichia coli preferentially utilises glucose before any other carbon source is called:

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Which of the following components of the pGLO plasmid is a reporter gene?

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With respect to the arabinose operon, the AraC protein exerts ______________ transcriptional control.

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Q11. The DNA sequences of the manR

promoter in the non-mutated strain of

A. melonitis

and Mutants A and B

are shown below. The -10 sequence is underlined, the transcription start site

is indicated in bold and the start codon is highlighted in yellow.

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Use the

sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)

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Q5.

RT-PCR

was performed on the enriched plant RNA using region-specific

primers for the

ProfX

gene. The RT-PCR products were analysed

by gel electrophoresis and the results are shown in the image below.

Which band (1, 2 or 3) corresponds with mRNA

transcript C?

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0%
100%
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Q11. The DNA sequences of the manR

promoter in the non-mutated strain of

A. melonitis

and Mutants A and B

are shown below. The -10 sequence is underlined, the transcription start site

is indicated in bold and the start codon is highlighted in yellow.

Use the

sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)

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Q10. What kind of protein is ManR likely to be?

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Q7. Which of the following statements apply to the regulation of catabolic operons? Select all that apply.

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