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MCB2011 - Molecular biology and the cell - S1 2025

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The ACTC1

gene encodes an actin protein that is only expressed in cardiomyocytes (i.e.

cardiac muscle cells). The schematic below shows the histone modifications that

are present in the

ACTC1

promoter region in cardiomyocytes vs

fibroblasts (i.e. skin cells).

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Use the

information provided above to explain why the

ACTC1

gene is actively expressed in

cardiomyocytes but not fibroblasts. (2-3 sentences) (2 marks)

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The ACTB

gene encodes an actin protein that is ubiquitously expressed in all human

cells. As shown in the schematic below, the promoter region of the

ACTB

gene is embedded within a CG island.

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The researcher

uses a specialised laboratory technique to examine DNA methylation in the

ACTB

promoter region. What do you hypothesise the researcher will find? (1 mark)

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In this laboratory class you will handle glass slides and coverslips. How should you dispose of these items?

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Which of the following GHS pictograms are listed in the Safety Data Sheet (SDS) for Triton X-100? Select all that apply.

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Which of the following reagents cause eye irritation and/or eye damage? Select all that apply.

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What personal protective equipment are you required to bring / wear to this class? Select all that apply.

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Which of the following reagents are fluorescent and thus light-sensitive? Select all that apply

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The genetically modified TOMM20-GFP HeLa cell line has GFP labelled:

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