The import of acyl-CoA dehydrogenase into the mitochondrion is defective

in cell line HEP-M1. The researcher used subcellular fractionation and western

blotting to detect acyl-CoA dehydrogenase in different subcellular

compartments. The western blotting results for normal healthy hepatocytes and the

HEP-M1 cell line are summarised in the figure below.

Which of the

following mutations (A, B, C) is most likely present in the HEP-M1 cell line?

Use the western blotting results and your knowledge of the mitochondrial

protein import process to justify your response. (2-3 sentences) (2 marks)

A. The acyl-CoA

dehydrogenase mitochondrial signal sequence is mutated

Acyl-CoA dehydrogenase is encoded in the nuclear genome, synthesised by

free ribosomes in the cytoplasm, and then imported into the mitochondrial

matrix. Acyl-CoA dehydrogenase is subject to which type(s) of intracellular

transport? (select all that apply)

A researcher extracts protein from cultured hepatocytes (i.e. liver

cells) and treats the protein sample with sodium dodecyl sulphate (SDS) and

β-mercaptoethanol (BME). The protein sample is loaded onto a polyacrylamide gel

and a western blot is performed as per the steps described in the relevant online

learning module (OLM 6.2). The researcher uses a primary antibody that specifically

binds to the N-terminal region of acyl-CoA dehydrogenase.

Examine the

western blotting results shown in the image above (A, B, C). Which result would

you expect to obtain for this experiment?

Which of the following techniques was likely used to elucidate the three-dimensional

structure of the acyl-CoA dehydrogenase homotetramer?

What structural motifs can you identify in the interactive ribbon model

of the acyl-CoA dehydrogenase homotetramer? (select all that apply)

The ribbon model of the acyl-CoA dehydrogenase homotetramer shown above depicts:

Which of the following hazard statements apply to the Assay Probe

(sodium dodecyl sulfate, 10% solution)? Select all that apply. HINT: You

need to search this chemical on the Chemwatch database.

The promoter that controls transcription of the arabinose operon is called:

When glucose is abundant the Catabolite Activator Protein (CAP):