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MCB2011 - Molecular biology and the cell - S1 2025

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Q9.

Based on the data in the above graphs, in which

mutant is carbon catabolite repression abolished? Briefly justify your response. (2-3 sentences) (2 marks)

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Q8.

Which of the following

molecules are involved in the global regulation of catabolic operons? Select

all that apply.

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Q6.The first 10 nucleotides of the first exon of the ProfX gene are 5’ ATG GGT TCC T 3’. What is the sequence of the first 10 nucleotides of transcribed mRNA in the pre-mRNA transcript?

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Q4. What type of splicing most likely produced mRNA transcript B?

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Q5.

RT-PCR

was performed on the enriched plant RNA using region-specific

primers for the

ProfX

gene. The RT-PCR products were analysed

by gel electrophoresis and the results are shown in the image below.

Image failed to load

Which band (1, 2 or 3) corresponds with mRNA

transcript C?

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Q3. What type of splicing most likely produced mRNA transcript A?

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Q2.

The researcher treated the

second batch of RNA with a MIXrobe kit that removes poly-adenylated RNA

transcripts using magnetic beads. Explain

why the MIXrobe kit does not remove bacterial mRNA. (1-2 sentences) (2 marks)

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Q1. 

The researcher treated one batch

of the extracted RNA with the reagent Captcha which specifically binds to

7-methylguanosine. 

Will Captcha bind to the plant RNA, A. melonitis

RNA, or

both? Briefly justify your response. (1-2 sentences) (2 marks)

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What personal protective equipment are you required to bring / wear to this practical? Select all that apply.

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Which of the following reagents can cause eye irritation and/or eye damage? Select all that apply.

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