Which of the following labels on the E2.5 embryo is incorrect?

In this practical apoptotic cells will be visualised using which dye?

How long does it take for a chick to go from fertilisation to hatching?

During the phylotypic stage, vertebrate embryos from different species ______________________________.

The import of acyl-CoA dehydrogenase into the mitochondrion is defective

in cell line HEP-M1. The researcher used subcellular fractionation and western

blotting to detect acyl-CoA dehydrogenase in different subcellular

compartments. The western blotting results for normal healthy hepatocytes and the

HEP-M1 cell line are summarised in the figure below.

Which of the

following mutations (A, B, C) is most likely present in the HEP-M1 cell line?

Use the western blotting results and your knowledge of the mitochondrial

protein import process to justify your response. (2-3 sentences) (2 marks)

A. The acyl-CoA

dehydrogenase mitochondrial signal sequence is mutated

A researcher extracts protein from cultured hepatocytes (i.e. liver

cells) and treats the protein sample with sodium dodecyl sulphate (SDS) and

β-mercaptoethanol (BME). The protein sample is loaded onto a polyacrylamide gel

and a western blot is performed as per the steps described in the relevant online

learning module (OLM 6.2). The researcher uses a primary antibody that specifically

binds to the N-terminal region of acyl-CoA dehydrogenase.

Examine the

western blotting results shown in the image above (A, B, C). Which result would

you expect to obtain for this experiment?

Which proteomic

technique would you use to compare the protein profiles of the two samples? Explain

why your chosen technique is suitable for this specific application. (2-3

sentences) (2 marks)

A signal sequence at the N-terminus of the albumin protein directs it to

the correct subcellular compartment following translation. The albumin signal

sequence likely contains: