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MCB2011 - Molecular biology and the cell - S1 2026
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Which of the following hazard statements are listed in the mini SDS for the Precision Plus Kaleidoscope Protein Standard (CW No. 3823559)? (select all that apply)
Following protein transfer, why is the polyvinylidene difluoride (PVDF) membrane blocked prior to immunodetection?
What is the polyvinylidene difluoride (PVDF) membrane immersed in to activate it?
During SDS–PAGE, all proteins migrate toward the positively charged electrode (anode). Why does this occur?
Laemmli buffer is classified as a highly hazardous chemical solution. Which of the following GHS symbols apply to this reagent? (select all that apply)
β-mercaptoethanol is a component of Laemmli buffer. What is the function of β-mercaptoethanol?
The MFN2 protein is comprised of six functional domains. Which domain is responsible for anchoring MFN2 in the outer mitochondrial membrane?
What is the approximate molecular weight of the full-length MFN2 protein in humans?
To study the processing of pre-mRNA transcripts, the researcher cultured human myocytes in the laboratory and used a technique called ultracentrifugation to isolate myocyte nuclei, ribosomes and cytoplasm.
Which isolated sample (nuclei, ribosomes or cytoplasm) would contain the greatest abundance of pre-mRNA transcripts? Briefly justify your response. (1-2 sentences) (2 marks)
To determine which aspect of carbon catabolism was impacted by mutagenesis in each of these strains, the strains were also assayed for carbon catabolism of glucose, xylose and arabinose and sent for genetic sequencing. It was found that PET #X had a mutation that resulted in constitutive CAP binding to the CAP binding site. Which of the graphs below is most likely to represent strain PET #X?