How long will the PVDF membrane be incubated in the anti-MFN2 antibody working solution?

Use the dilution equation (C1V1 = C2V2) to calculate the volume (µL) of anti-MFN2 antibody that is needed to prepare 5mL of a 0.2µg/mL working solution.

Which type of detection method will be used in this laboratory to visualise MFN2 on the PVDF membrane?

You will use an anti-MFN2 antibody to probe the PVDF membrane and detect the MFN2 protein during western blotting. The anti-MFN2 antibody used in this laboratory is conjugated to:

Which of the following reagents are toxic in contact with the skin? (select all that apply)

Which of the following hazard statements are listed in the mini SDS for the Precision Plus Kaleidoscope Protein Standard (CW No. 3823559)? (select all that apply)

Following protein transfer, why is the polyvinylidene difluoride (PVDF) membrane blocked prior to immunodetection?

What is the polyvinylidene difluoride (PVDF) membrane immersed in to activate it?

During SDS–PAGE, all proteins migrate toward the positively charged electrode (anode). Why does this occur?

Laemmli buffer is classified as a highly hazardous chemical solution. Which of the following GHS symbols apply to this reagent? (select all that apply)