Chemistry and Biochemistry
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Determine the type of hyperbilirubinemia if the following values have been obtained:
serum
|
urine
| ||
total bilirubin
|
direct bilirubin
|
total bilirubin
|
direct bilirubin
|
57 μmol/L
|
44 μmol/L
|
+
|
-
|
Substances formed during the degradation of heme are called:
As the name suggests, these are colored substances. Assign to each of them its colour:
Biliverdin
|
|
Bilirubin
|
|
Urobilinogen
|
|
Bilicyanin
|
|
Stercobilinogen
|
|
Xanthine-oxidase is an enzyme, which participate in:
FAD is coenzyme of:
The substance acts in a reaction catalyzed by xanthine oxidase (the
reaction will be done in our laboratory classes) as:
What type of inhibition (competitive / non-competitive) the given
statement applies to.
The figure shows the course of the enzymatically catalyzed reaction.
Determine the type of hyperbilirubinemia if the following values have been obtained:
serum
|
urine
| ||
total bilirubin
|
direct bilirubin
|
total bilirubin
|
direct bilirubin
|
72 μmol/L
|
41 μmol/L
|
+
|
+
|
The so-called direct bilirubin and total bilirubin are estimated. The reagent is diazotized sulfanilic acid, reaction with bilirubin produces an azo dye, the absorbance of which is measured.
Direct bilirubin reacts directly with the reagent, we must use an accelerator to determine the total bilirubin.
The accelerator ensures:
Thus, direct bilirubin is bilirubin:
Indirect bilirubin is:
indirect bilirubin level = total bilirubin - direct bilirubin
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