MCB2011 - Molecular biology and the cell - S1 2025
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The biomedical
researcher discovers that the defective acyl-CoA dehydrogenase subunits in the HEP-M2
cell line are targeted for proteasomal degradation. Which post-translational
modification is likely responsible for targeting acyl-CoA dehydrogenase to the
proteasome?
Explain why the mutation in the HEP-M2 cell line completely disrupts the
assembly of the acyl-CoA dehydrogenase homotetramer, whereas the mutation in
the HEP-M3 cell line has no impact on the assembly or structure of the
homotetramer. (2-3 sentences) (2 marks)
The import of acyl-CoA dehydrogenase into the mitochondrion is defective in cell line HEP-M1. The researcher used subcellular fractionation and western blotting to detect acyl-CoA dehydrogenase in different subcellular compartments. The western blotting results for normal healthy hepatocytes and the HEP-M1 cell line are summarised in the figure below.
Which of the following mutations (A, B, C) is most likely present in the HEP-M1 cell line? Use the western blotting results and your knowledge of the mitochondrial protein import process to justify your response. (2-3 sentences) (2 marks)
A. The acyl-CoA dehydrogenase mitochondrial signal sequence is mutated B. The receptor protein in the TOM complex is mutated C. The translocation channel in the TIM23 complex is mutated
Acyl-CoA dehydrogenase is encoded in the nuclear genome, synthesised by free ribosomes in the cytoplasm, and then imported into the mitochondrial matrix. Acyl-CoA dehydrogenase is subject to which type(s) of intracellular transport? (select all that apply)
A researcher extracts protein from cultured hepatocytes (i.e. liver cells) and treats the protein sample with sodium dodecyl sulphate (SDS) and β-mercaptoethanol (BME). The protein sample is loaded onto a polyacrylamide gel and a western blot is performed as per the steps described in the relevant online learning module (OLM 6.2). The researcher uses a primary antibody that specifically binds to the N-terminal region of acyl-CoA dehydrogenase.
Examine the western blotting results shown in the image above (A, B, C). Which result would you expect to obtain for this experiment?
Which of the following techniques was likely used to elucidate the three-dimensional structure of the acyl-CoA dehydrogenase homotetramer?
What structural motifs can you identify in the interactive ribbon model of the acyl-CoA dehydrogenase homotetramer? (select all that apply)
The ribbon model of the acyl-CoA dehydrogenase homotetramer shown above depicts:
Which of the following hazard statements apply to the Assay Probe (sodium dodecyl sulfate, 10% solution)? Select all that apply. HINT: You need to search this chemical on the Chemwatch database.
The promoter that controls transcription of the arabinose operon is called:
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