MCB2011 - Molecular biology and the cell - S1 2025
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Q7. Which of the following statements apply to the regulation of catabolic operons? Select all that apply.
Based on the data in the above graphs, in which
mutant is carbon catabolite repression abolished? Briefly justify your response. (2-3 sentences) (2 marks)
Q8. Which of the following molecules are involved in the global regulation of catabolic operons? Select all that apply.
Q6.The first 10 nucleotides of the first exon of the ProfX gene are 5’ ATG GGT TCC T 3’. What is the sequence of the first 10 nucleotides of transcribed mRNA in the pre-mRNA transcript?
Q4. What type of splicing most likely produced mRNA transcript B?
Q5. RT-PCR was performed on the enriched plant RNA using region-specific primers for the gene. The RT-PCR products were analysed by gel electrophoresis and the results are shown in the image below.
Which band (1, 2 or 3) corresponds with mRNA
transcript C?
Q3. What type of splicing most likely produced mRNA transcript A?
Q2. The researcher treated the second batch of RNA with a MIXrobe kit that removes poly-adenylated RNA transcripts using magnetic beads. Explain why the MIXrobe kit does not remove bacterial mRNA. (1-2 sentences) (2 marks)
Q1. The researcher treated one batch of the extracted RNA with the reagent Captcha which specifically binds to 7-methylguanosine. RNA, or both? Briefly justify your response. (1-2 sentences) (2 marks)
What personal protective equipment are you required to bring / wear to this practical? Select all that apply.
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