MCB2011 - Molecular biology and the cell - S1 2025
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When glucose is abundant the Catabolite Activator Protein (CAP):
When setting up the colorimetric assay, in what order should the sample and reagents be added to each well?
The assay probe has been provided at a stock concentration of 50µg/mL. Calculate how many µL of probe must be diluted to prepare 1mL of a 1µg/mL solution.
The phenomenon whereby Escherichia coli preferentially utilises glucose before any other carbon source is called:
Which of the following components of the pGLO plasmid is a reporter gene?
With respect to the arabinose operon, the AraC protein exerts ______________ transcriptional control.
Q11. The DNA sequences of the manR promoter in the non-mutated strain of and Mutants A and B are shown below. The -10 sequence is underlined, the transcription start site is indicated in bold and the start codon is highlighted in yellow.
Use the sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)
Q5. RT-PCR was performed on the enriched plant RNA using region-specific primers for the gene. The RT-PCR products were analysed by gel electrophoresis and the results are shown in the image below.
Which band (1, 2 or 3) corresponds with mRNA
transcript C?
Q11. The DNA sequences of the manR promoter in the non-mutated strain of and Mutants A and B are shown below. The -10 sequence is underlined, the transcription start site is indicated in bold and the start codon is highlighted in yellow.
Use the sequencing data above to explain why mannose is not metabolised by Mutant B? (2-3 sentences) (2 marks)
Q10. What kind of protein is ManR likely to be?
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