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DEV3011 - Experimental developmental biology and disease modelling - S1 2026
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ENU mutagenesis in mice is a forward genetic screen.
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What happens to LoxP sites in the presence of Cas9?
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Post-doctoral researcher Sandeep wants to delete the Fgf8 gene in mouse embryos and see what effect this has in the limbs. However, global deletion of Fgf8 results in embryo lethality before the limbs form. What alternative approach could he use to achieve his aim?
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What is traditionally used for a tamoxifen-mediated inducible conditional knock-out in mouse?
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In making a knockout mouse, what occurs during homologous recombination?
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Sarah is a PhD student who wants to mis-express the Growth Hormone (GH) gene throughout the entire mouse embryo, using transgenic pronuclear injection. What does she need for this experiment?
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Conditional deletion of the Sonic hedgehog (Shh) gene in the neural tube of the mouse embryo is an example of a forward genetic screen.
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True or False In the zebrafish, CRISPR/Cas9 can be used for gene knock-in.
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In the image below of the CRISPR/Cas9 system, what does the black arrow denote?
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What happens when cytoplasmic beta-catenin is not degraded in the cell?
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