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DEV3011 - Experimental developmental biology and disease modelling - S1 2026
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What determines which cells of the early mouse embryo form inner cell mass vs outer trophoblast?
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You decide to make a mouse reporter model expressing GFP in the neural tube, using the traditional knock-in strategy. What is a part of this method? Choose the best option below.
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Two morphogens are produced along the anterior-posterior axis of the Drosophila embryo.
Morphogen 1 is expressed most highly at the anterior pole, tapering with distance from its source.
Morphogen 2 is expressed highly at the posterior pole, also tapering with distance from its source.
Expression of Gene 3 is stimulated only by intermediate levels of morphogen 1.
Expression of Gene 4 is inhibited by any expression of gene 3 and morphogen 2.
Expression of Gene 5 is stimulated only by intermediate and high levels of morphogen 1.
Expression of morphogen 2 is inhibited by any level of gene 5 expression.
In the figure above, the arrow denotes the expression of which gene or morphogen?
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A cell carries a mutation that renders the GSK3 protein inactive. What would be the result?
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What is the significance of cortical rotation in the frog embryo?
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You plan to insert a gene into the genome of zebrafish embryos, using CRISPR/Cas9. What would NOT typically be involved in your approach?
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What organising centre in the Xenopus embryo is the functional equivalent of Hensen’s node in chick?
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Inducible knockout mouse models permit temporal control over the induction of specific gene knockouts. Tamoxifen is commonly used to induce Cre-mediated recombination in mouse models. How does tamoxifen act?
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What is the result of the FLP-FRT system used in Drosophila?
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Epidermal growth factor secreted by a cell can bind to its receptor on that cell. This is an example of what developmental process?
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