Refer to the expected

results shown in Figure 1. Compare the appearance of the mitochondrial network

in the wild-type and patient-derived fibroblasts. Describe any observable

differences in mitochondrial morphology or network structure. What might these

differences indicate about mitochondrial fusion? (2-3 sentences) (3 marks)

Refer to the expected

results shown in Figure 1. Compare the cell density and overall cellular

morphology of the wild-type fibroblasts and the patient-derived fibroblasts.

Describe any observable differences in cell shape, size, or density. What might

these differences indicate about the health of the cells? (2-3 sentences) (3

marks)

The import of acyl-CoA dehydrogenase into the mitochondrion is defective

in cell line HEP-M1. The researcher used subcellular fractionation and western

blotting to detect acyl-CoA dehydrogenase in different subcellular

compartments. The western blotting results for normal healthy hepatocytes and the

HEP-M1 cell line are summarised in the figure below.

Which of the

following mutations (A, B, C) is most likely present in the HEP-M1 cell line?

Use the western blotting results and your knowledge of the mitochondrial

protein import process to justify your response. (2-3 sentences) (2 marks)

A. The acyl-CoA

dehydrogenase mitochondrial signal sequence is mutated

The HEP-M2 cell lines carries a mutation in the acyl-CoA

dehydrogenase subunit interface domain (SID). SID mediates inter-subunit

interactions and forms the internal core of the acyl-CoA dehydrogenase homotetramer. An excerpt of the amino acid sequence of the SID domain for normal healthy hepatocytes compared to HEP-M2 cells is shown below:

• Normal: -Leu-Val-Ala-Ala-Ile-
• HEP-M2: -Leu-Val-Gln-Ala-Ile-

Which of the following reagents can be fatal if swallowed? (select all that apply)

Which of the following hazard statements are listed in the mini SDS for Triton X-100 (CW No. 12977) (select all that apply)

Triton X-100 is classified as a hazardous chemical. Which of the following GHS symbols apply to this reagent? (select all that apply)

In this laboratory class you will handle glass multiwell slides and glass coverslips. How should you discard these items?

Which of the following reagents are fluorescent and thus light-sensitive? (select all that apply)

Use the dilution equation (C₁V₁ = C₂V₂) to calculate the volume (µL) of 200U/mL Phalloidin AF488 that is needed to prepare 150µL of a 3U/mL working solution.