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MCB2011 - Molecular biology and the cell - S1 2025
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Q1. Match the molecular tool to the experimental need
SCCs treated with dekjensine were stained with an anti-cytochrome-C GFP-tagged antibody and a reagent called mitotracker that labels mitochondria with RFP. The fluorescent images below show the results for an untreated SCC and a
Interpret the fluorescent images to select the correct statement:
The researcher
captured the electron micrograph image below which shows the release of albumin
from a secretory vesicle within a hepatocyte into the extracellular
environment.
Identify the donor and target membranes for the secretory vesicle. (2
marks)
The import of acyl-CoA dehydrogenase into the mitochondrion is defective in cell line HEP-M1. The researcher used subcellular fractionation and western blotting to detect acyl-CoA dehydrogenase in different subcellular compartments. The western blotting results for normal healthy hepatocytes and the HEP-M1 cell line are summarised in the figure below.
Which of the following mutations (A, B, C) is most likely present in the HEP-M1 cell line? Use the western blotting results and your knowledge of the mitochondrial protein import process to justify your response. (2-3 sentences) (2 marks)
A. The acyl-CoA dehydrogenase mitochondrial signal sequence is mutated B. The receptor protein in the TOM complex is mutated C. The translocation channel in the TIM23 complex is mutated
Which of the following ultrastructural changes would you expect to observe in cells treated with dekjensine? Select all that apply.
SCCs treated with dekjensine were stained with an anti-cytochrome-C GFP-tagged antibody and a reagent called mitotracker that labels mitochondria with RFP. The fluorescent images below show the results for an untreated SCC and a
Interpret the fluorescent images to select the correct statement:
Which method could the researcher use to detect cells within the S-phase of the cell cycle?
Activation of quiescent cancer stem cells can cause metastatic relapse in cancer patients. Do you expect quiescent cancer stem cells to be susceptible or resistant to ? Briefly justify your response. (2-3 sentences) (2 marks)
The researcher tested the efficacy of a new chemotherapeutic drug named
dekjensinethat specifically targets cells in the S-phase of the cell
cycle by inhibiting DNA synthesis. In the laboratory, SCCs treated with the
drug undergo cell cycle arrest and cell death.
Which cell cycle checkpoint would the treated SCCs arrest at?
Briefly justify your response. (2-3 sentences) (2 marks)
A biomedical researcher monitored cell division in SCCs cultured in the laboratory. The researcher immunostained the cells with DAPI to label DNA and a fluorescently tagged __________ to label the mitotic spindle.